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氣管肺泡灌洗液 ( bronchoalveolar lavage fluid, BALF)流式檢測分群圈門策略

更新時間：2024-09-09 點擊次數：1412次

Carbapenem-resistant Klebsiella pneumoniae (CRKP)感染小鼠模型，氣管肺泡灌洗液 ( bronchoalveolar lavage fluid, BALF)流式檢測分群圈門策略，可以參考下圖。單核巨噬清除訂購信息見后。

Gating strategy to characterize immune cells recovered in BALF. BALF cells were gated for live cells by excluding LIVE/DEAD fixable aqua-stained cells.

Leukocytes were gated as CD45+cells, which were further separated into Siglec-F+, CD11b+ and CD11b– cells. Siglec-F+ (SF+) cells were further gated into F4/80+ and CD11c+; both F4/80+ and CD11c+ cells were identified as alveolar macrophages (AMs). From CD11b+ cells, Ly6G+ cells were gated as neutrophils. CD11b+ Ly6G– cells were gated for interstitial macrophages (IMs) (CD11b+ F4/80+). Remaining cell populations were separated into Ly6Chi monocytes (CD11b+ Ly6Chi) and Ly6Clo monocytes (CD11b+ Ly6Clo). CD11b– cells were gated into B cells (B220+ cells) and remaining CD11b– B220–cells were separated into CD4+ T cells, CD8+ T cells, CD4+ CD8+ T cells and CD4– CD8– T cells(non-T & -B cells).

品牌：Liposoma

產地：荷蘭

貨號：CP-005-005

規格：5ml+5ml

品名：Clodronateliposomes & Control Liposomes

代理：靶點科技

部位：肺臟

細胞：肺泡巨噬細胞（AM）

劑量：70ul

方式：滴鼻（intratracheal administration）

時間點：建模前48h

題目：Lung-innervating nociceptor sensory neurons promote pneumonic sepsis during carbapenem-resistant Klebsiella pneumoniae lung infection